تجزیه ﺑﻴﺎن ﻣﺘﻔﺎوت ژنﻫﺎ در ﺑﺮﻫﻢ ﻛﻨﺶ ﮔﻨﺪم- Mycosphaerella graminicola

Septoria leaf blotch (STB) caused by Mycosphaerella graminicola is one of the most important diseases in different regions of world. In Iran, STB has intensified in recent years because of extensive culture of susceptible cultivars. The use of resistant cultivars is the most effective method of controlling STB. The use of resistant cultivars is the most effective method of controlling STB. For successful breeding of resistant cultivars, our knowledge about genes related to STB resistance is necessary. In this study, cDNA-AFLP analysis was used to identify genes involved in resistance to STB. Resistant cultivar Wangshuibai and susceptible cultivar Falat were incubated with M. graminicola in the greenhouse, and then were sampled at 11 time points. Using 37 primer combinations, 2200 fragments were observed. 59 Transcript-derived fragments that showed differential expression after inoculation were identified, sequenced and characterized. Finally, 32 ESTs related to STB were submitted at NCBI databank. This results determined some genes involved in STB resistance in wheat during infection which can help to elucidate genetics basis of resistance.