اثر سیلیکون بر فعالیت آنزیمهای آسکوربات پراکسیداز و گوآیکول پراکسیداز تحت تنش خشکی

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عنوان دوره: سیزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
1داﻧﺸﺠﻮی ﻛﺎرﺷﻨﺎﺳﻲ ارﺷﺪ ﮔﺮوه ﺑﻴﻮﺗﻜﻨﻮﻟﻮژی ﻛﺸﺎورزی داﻧﺸﮕﺎه ﺑﻴﻦ اﻟﻤﻠﻠﻲ اﻣﺎم ﺧﻤﻴﻨﻲ (ره)
2داﻧﺸﻴﺎر، ﮔﺮوه ﺑﻴﻮﺗﻜﻨﻮﻟﻮژی ﻛﺸﺎورزی داﻧﺸﮕﺎه ﺑﻴﻦ اﻟﻤﻠﻠﻲ اﻣﺎم ﺧﻤﻴﻨﻲ
3داﻧﺸﻴﺎر ﮔﺮوه ﺑﻴﻮﺗﻜﻨﻮﻟﻮژی ﻛﺸﺎورزی داﻧﺸﮕﺎه ﺑﻴﻦ اﻟﻤﻠﻠﻲ اﻣﺎم ﺧﻤﻴﻨﻲ
4اﺳﺘﺎدﻳﺎر ﮔﺮوه ﺑﻴﻮﺗﻜﻨﻮﻟﻮژی ﻛﺸﺎورزی داﻧﺸﮕﺎه ﺑﻴﻦ اﻟﻤﻠﻠﻲ اﻣﺎم ﺧﻤﻴﻨﻲ
چکیده
ﻜﻴﺪه
ﺳﻴﻠﻴﻜﻮن دوﻣﻴﻦ ﻋﻨﺼﺮ ﻣﻔﻴﺪ ﺑﺮای رﺷﺪ ﮔﻴﺎﻫﺎن ﺷﻨﺎﺧﺘﻪ ﺷﺪه اﺳﺖ. در ﺑﺴﻴﺎری از ﺑﺮرﺳﻲﻫﺎ ﺑﻪ اﺛﺮات ﻣﻔﻴﺪ ﺳﻴﻠﻴﻜﻮن در ﺣﺎﺻﻠﺨﻴﺰی ﺧﺎک و رﺷﺪ ﮔﻴﺎﻫﺎن اﺷﺎره ﺷﺪه اﺳﺖ. ﺳﺎﻟﺴﻴﺴﻠﻴﻚ اﺳﻴﺪ ﺗﺎﺛﻴﺮ ﺑﺴﻴﺎر ﻣﻬﻤﻲ در رﺷﺪ و اﻓﺰاﻳﺶ ﻣﻘﺎوﻣﺖ ﮔﻴﺎﻫﺎن ﺑﻪ ﺗﻨﺶ ﺧﺸﻜﻲ دارد. ﺑﺪﻳﻦ ﻣﻨﻈﻮر اﺛﺮ ﻧﺎﺷﻲ از ﺗﻨﺶ ﺧﺸﻜﻲ در ﻓﻌﺎﻟﻴﺖ آﻧﺰﻳﻢﻫﺎی ﺿﺪاﻛﺴﻨﺪه آﺳﻜﻮرﺑﺎت ﭘﺮاﻛﺴﻴﺪاز و ﮔﻮآﻳﻜﻮل ﭘﺮاﻛﺴﻴﺪاز در دو ﻻﻳﻦ ﮔﻴﺎه ﺟﻮ دوردﻳﻔﻪ (CB -20315 (Hordeum vulgare L (ﻣﻘﺎوم) و CB-20213 (ﺣﺴﺎس) در ﻣﺮﺣﻠﻪ ﭼﻬﺎر ﺑﺮﮔﻲ در ﮔﻠﺨﺎﻧﻪ ﻣﻮرد ﺑﺮرﺳﻲ ﻗﺮار ﮔﺮﻓﺖ. آزﻣﺎﻳﺶ ﺑﻪ ﺻﻮرت ﻓﺎﻛﺘﻮرﻳﻞ در ﻗﺎﻟﺐ ﻃﺮح ﻛﺎﻣﻼ ﺗﺼﺎدﻓﻲ ﺑﺎ ﺳﻪ ﺗﻜﺮار و ﺳﻪ ﺗﻴﻤﺎر ﺷﺎﻫﺪ، ﺧﺸﻜﻲ و ﺳﻴﻠﻴﻜﻮن– ﺧﺸﻜﻲ )2ﻣﻴﻠﻲﻣﻮﻻر ﺳﻴﻠﻴﻜﺎتﺳﺪﻳﻢ/ ﻛﻴﻠﻮﮔﺮم ﺧﺎک( اﺟﺮا ﺷﺪ. ﻧﺘﺎﻳﺞ ﻧﺸﺎن داد ﻛﻪ در ﻫﺮ دو رﻗﻢ ﻛﺎرﺑﺮد ﺳﻴﻠﻴﻜﻮن، ﻓﻌﺎﻟﻴﺖ آﻧﺰﻳﻢﻫﺎی ﺿﺪ اﻛﺴﻨﺪه را ﺗﺤﺖ ﺷﺮاﻳﻂ ﺗﻨﺶ ﺧﺸﻜﻲ اﻓﺰاﻳﺶ ﻣﻲدﻫﺪ. ﺑﻨﺎﺑﺮاﻳﻦ ﺳﻴﻠﻴﻜﻮن ﺑﺎﻋﺚ ﻛﺎﻫﺶ ﺧﺴﺎرت اﻛﺴﻴﺪاﺗﻴﻮ ﻧﺎﺷﻲ از ﮔﻮﻧﻪﻫﺎی ﻓﻌﺎل اﻛﺴﻴﮋن ﻣﻲﺷﻮد و از ﻓﺮآﻳﻨﺪﻫﺎی ﻓﻴﺰﻳﻮﻟﻮژﻳﻜﻲ ﮔﻴﺎﻫﺎﻧﻲ ﻛﻪ در ﻣﻌﺮض ﺗﻨﺶ ﻗﺮار ﮔﺮﻓﺘﻪاﻧﺪ ﻣﺤﺎﻓﻈﺖ ﻣﻲﻛﻨﺪ.
کلیدواژه ها
 
Title
Effects of silicon on enzymatic activity of ascorbate peroxidase and guaiacol peroxidase under drought
Authors
Abstract
Silicon is the second most beneficial element in plant growth. Most of the studies are discussed on the beneficial effects of silicon in soil fertility and plant growth. Salicylic acid has a very important impact on the growth and increase the resistance of plants to drought stress. Accordingly, Effect of drought stress on antioxidant enzymes activities, ascorbate peroxidase and guaiacol peroxidase was investigated in two line of two-row barley named CB-20315(resistant) and CB-20213 (sensitive) in four leaves stage in greenhouse. The experiment preformed in randomized factorial design in three treatments, control, drought and silicon-drought (sodium silicate 2 mg / 1kg), with three replication. The results showed that in both varieties, silicon application increased activity of antioxidant enzymes under drought stress. Therefore silicon reduces oxidative damage induced by reactive oxygen species and protects physiological processes in plants under stress.
Keywords
Antioxidant Enzymes, drought stress, Silicon, Barley