بررسی اثر سیلیکون بر آنزیمهای ضداکسنده در گیاه جو تحت تنش خشکی

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عنوان دوره: سیزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
1داﻧﺸﺠﻮی ﻛﺎرﺷﻨﺎﺳﻲ ارﺷﺪ ﺑﻴﻮﺗﻜﻨﻮﻟﻮژی ﮔﻴﺎﻫﻲ، داﻧﺸﮕﺎه ﺑﻴﻦاﻟﻤﻠﻠﻲ اﻣﺎم ﺧﻤﻴﻨﻲ (ره)
2داﻧﺸﻴﺎر داﻧﺸﮕﺎه ﺑﻴﻦاﻟﻤﻠﻠﻲ اﻣﺎم ﺧﻤﻴﻨﻲ (ره)
3داﻧﺸﻴﺎر داﻧﺸﮕﺎه ﺑﻴﻦاﻟﻤﻠﻠﻲ اﻣﺎم ﺧﻤﻴﻨﻲ (ره)
4اﺳﺘﺎدﻳﺎر داﻧﺸﮕﺎه ﺑﻴﻦاﻟﻤﻠﻠﻲ اﻣﺎم ﺧﻤﻴﻨﻲ (ره)
چکیده
ﺗﻨﺶ ﺧﺸﻜﻲ ﻳﻜﻲ از ﺑﺰرﮔﺘﺮﻳﻦ ﺗﻨﺶﻫﺎی ﻏﻴﺮ زﻧﺪه در ﺳﻄﺢ ﺟﻬﺎن اﺳﺖ. از ﻃﺮﻓﻲ ﺳﻴﻠﻴﻜﻮن دوﻣﻴﻦ ﻋﻨﺼﺮ راﻳﺞ در ﺧﺎک اﺳﺖ ﻛﻪ دارای اﺛﺮات ﻣﻔﻴﺪی در اﻓﺰاﻳﺶ ﺗﺤﻤﻞ ﺑﻪ ﺗﻨﺶﻫﺎی زﻧﺪه و ﻏﻴﺮ زﻧﺪه در ﮔﻴﺎﻫﺎن اﺳﺖ. ﻧﻘﺶ ﺳﻴﻠﻴﻜﻮن ﺧﺎرﺟﻲ در اﻓﺰاﻳﺶ ﻣﻘﺎوﻣﺖ ﮔﻴﺎه ﺑﻪ ﺗﻨﺶ ﻫﺎی ﻣﺨﺘﻠﻒ ﻣﺎﻧﻨﺪ ﺧﺸﻜﻲ، ﺷﻮری، ﺳﻤﻴﺖ ﻓﻠﺰی و ﺗﺸﻌﺸﻊ ﻓﺮاﺑﻨﻔﺶ اﺛﺒﺎت ﺷﺪه اﺳﺖ. ﻫﺪف از اﻳﻦ ﻣﻄﺎﻟﻌﻪ، ﺑﺮرﺳﻲ اﺛﺮ ﺳﻴﻠﻴﻜﻮن ﺑﺮ ﺗﻐﻴﻴﺮات ﻋﻤﻠﻜﺮدی دو آﻧﺰﻳﻢ ﺿﺪاﻛﺴﻨﺪهی ﺳﻮﭘﺮاﻛﺴﻴﺪ دﻳﺴﻤﻮﺗﺎز )SOD( و ﭘﺮاﻛﺴﻴﺪاز )POX( در ﮔﻴﺎه ﺟﻮ ﺗﺤﺖ ﺗﻨﺶ ﺧﺸﻜﻲ اﺳﺖ. آزﻣﺎﻳﺶ ﻓﻮق در ﻗﺎﻟﺐ ﻃﺮح ﻓﺎﻛﺘﻮرﻳﻞ ﺗﺼﺎدﻓﻲ در ﺳﻪ ﺗﻴﻤﺎر ﻛﻨﺘﺮل، ﺧﺸﻜﻲ و ﺳﻴﻠﻴﻜﻮن- ﺧﺸﻜﻲ و در ﺳﻪ ﺗﻜﺮار و ﻣﺮﺣﻠﻪی ﭼﻬﺎر ﺑﺮﮔﻲ ﺑﺮ روی دو رﻗﻢ ﺑﻪ ﻧﺎمﻫﺎی ﻧﻴﻤﺮوز )ﻣﻘﺎوم ﺑﻪ ﺧﺸﻜﻲ( و ﮔﺮﮔﺎن ﺣﺴﺎس( ﺑﻪ روشﻫﺎی اﺳﭙﻜﺘﺮوﻓﺘﻮﻣﺘﺮی و ژل ﺑﻮﻣﻲ اﻧﺠﺎم ﺷﺪ. ﻧﺘﺎﻳﺞ ﻧﺸﺎن داد ﻛﻪ ﺗﻴﻤﺎر ﺳﻴﻠﻴﻜﻮن در ﻣﺠﻤﻮع ﺑﺎﻋﺚ اﻓﺰاﻳﺶ در ﻣﻴﺰان ﻓﻌﺎﻟﻴﺖ اﻳﻦ دو آﻧﺰﻳﻢ ﺑﻮﻳﮋه آﻧﺰﻳﻢ ﺳﻮﭘﺮاﻛﺴﻴﺪ دﺳﻤﻮﺗﺎز در ﮔﻴﺎﻫﺎن ﺗﺤﺖ ﺗﻨﺶ ﺧﺸﻜﻲ ﺷﺪ. اﻟﺒﺘﻪ در رﻗﻢ ﻣﻘﺎوم اﻳﻦ اﻓﺰاﻳﺶ ﻧﺴﺒﺖ ﺑﻪ رﻗﻢ ﺣﺴﺎس ﻣﺸﻬﻮدﺗﺮ ﺑﻮد و اﻳﻦ اﻣﺮ ﻧﺸﺎن دﻫﻨﺪهی ﻛﺎﻫﺶ ﺧﺴﺎرتﻫﺎی اﻛﺴﻴﺪاﺗﻴﻮ ﻧﺎﺷﻲ از ﺗﻨﺶ ﺧﺸﻜﻲ از ﻃﺮﻳﻖ اﻓﺰاﻳﺶ ﻓﻌﺎﻟﻴﺖ آﻧﺰﻳﻢﻫﺎی ﺿﺪ اﻛﺴﻨﺪه ﺑﺮ روی ﮔﻴﺎه ﺟﻮ در ﺣﻀﻮر ﺳﻴﻠﻴﻜﻮن اﺳﺖ.
کلیدواژه ها
 
Title
Effect of silicon on anti-oxidation enzymes in barley plants under drought stress
Authors
Abstract
Drought stress is one of the largest abiotic stresses worldwide. Silicon is the second most abundant element in soils that have beneficial effects on biotic and abiotic stress tolerance in plants. Effect of external silicon in increasing plant resistance to various stresses such as drought, salinity, metal toxicity and UV radiation has been demonstrated. The purpose of this study is to investigate the effect of silicon on yield of two antioxidant enzymes named superoxide dismutase (SOD) and peroxidase (POX) in two barley cultivars, Nimruz (resistance) and Gorgan (sensitive), under drought stress. This experiment is performed in a randomized factorial design with three treatments (control, drought, and silicon-drought) with three replications in four leaves stage of barley (Hordeum vulgar L.) by spectrophotometry and native gel methods. The results showed that treatment of silicon increase the activity of these two enzymes especially superoxide dismutase enzyme. However, the increase in resistant cultivars was more than sensitive cultivar. These results represent a reduction of oxidative damage induced by drought stress by increasing antioxidant enzymes in presence of silicon on barley plant.
Keywords
drought stress, peroxidase, Silicon, superoxide dismutase