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عنوان دوره: پانزدهمین کنگره زراعت و اصلاح نباتات ایران
نویسندگان
Naeem Khan1 ، Bano Asghari1 ، ali babar2 ، Morteza Siavoshi3 ، Peiman Zandi4 ، Katarzyna Możdżeń5 ، Saikat Kumar Basu6
1Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan
23Department of Agronomy, University of Florida, USA
3Department of Agricultural Science, Payame Noor University, I. R. of Iran *corresponding and presenting
4Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, P. R. China
5Institute of Biology, Pedagogical University of Cracow, ul. Podchorążych 2, 30-084 Krakow
6Department of Biological Sciences, University of Lethbridge, Lethbridge, AB T1K3M4, Canada
چکیده
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کلیدواژه ها
Title
Induction of drought tolerance in chickpea (Cicer arietinum L.) gown in
sandy soil and modulation of plant metabolites as affected by PGR and
PGPR
Authors
Abstract
The plant growth promoting rhizobacteria (PGPR) can be implicated to utilize soils of low or marginal
productivity for crop production. The present study was carried out to investigate the role of PGPR
and PGRs (plant growth regulators) on the physiology of chickpea grown in sandy soil. The results
showed that plants treated with consortium of PGPR and PGRS significantly enhanced the
chlorophyll, protein and sugar contents. Highly significant increases (77%) were recorded for relative
water content in PGPR and PGRs treated plants. Leaf proline content, lipid peroxidation and activities
of antioxidant enzymes (CAT, APOX, POD and SOD) were increased in response to drought stress
but decreased due to PGPR. Grain weight (41%), number of nodules (78%), pod weight (53%) and
total biomass (54%) were higher in PGPR and PGR treated plants grown in sandy soil. Proline, Larginine,
L-histidine, L-isoleucine and tryptophan were accumulated in the leaves of chickpea exposed
to drought stress. Consortium of PGPR and PGRs induced significant accumulation of riboflavin, Lasparagine,
aspartate, glycerol, nicotinamide, and 3-hydroxy-3-methyglutarate in leaves of chickpea.
Sensitive genotype showed significant accumulation of nicotinamide and 4-hydroxy-methylglycine in
PGPR and PGR treated plants at both time points (44 and 60 days) as compared to non-inoculated
drought plants. Arginine accumulation was also enhanced in the leaves of sensitive genotype under
drought condition. Based on classical univariate and multivariate ROC curve analysis, thirteen
metabolites with high AUC values Area under receiver operating characteristic (ROC) curve, >0.964)
were identified as potential biomarkers for drought tolerance. Integrative use of consortia of PGPR
and SA appears to be an effective eco-friendly approach to induce drought tolerance in crop plants.
productivity for crop production. The present study was carried out to investigate the role of PGPR
and PGRs (plant growth regulators) on the physiology of chickpea grown in sandy soil. The results
showed that plants treated with consortium of PGPR and PGRS significantly enhanced the
chlorophyll, protein and sugar contents. Highly significant increases (77%) were recorded for relative
water content in PGPR and PGRs treated plants. Leaf proline content, lipid peroxidation and activities
of antioxidant enzymes (CAT, APOX, POD and SOD) were increased in response to drought stress
but decreased due to PGPR. Grain weight (41%), number of nodules (78%), pod weight (53%) and
total biomass (54%) were higher in PGPR and PGR treated plants grown in sandy soil. Proline, Larginine,
L-histidine, L-isoleucine and tryptophan were accumulated in the leaves of chickpea exposed
to drought stress. Consortium of PGPR and PGRs induced significant accumulation of riboflavin, Lasparagine,
aspartate, glycerol, nicotinamide, and 3-hydroxy-3-methyglutarate in leaves of chickpea.
Sensitive genotype showed significant accumulation of nicotinamide and 4-hydroxy-methylglycine in
PGPR and PGR treated plants at both time points (44 and 60 days) as compared to non-inoculated
drought plants. Arginine accumulation was also enhanced in the leaves of sensitive genotype under
drought condition. Based on classical univariate and multivariate ROC curve analysis, thirteen
metabolites with high AUC values Area under receiver operating characteristic (ROC) curve, >0.964)
were identified as potential biomarkers for drought tolerance. Integrative use of consortia of PGPR
and SA appears to be an effective eco-friendly approach to induce drought tolerance in crop plants.
Keywords
Growth regulators, UPLC-HRMS, EPS, metabolites, ROC cure analysis, drought tolerance